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Fig. 1 | Molecular Medicine

Fig. 1

From: Insulin-Stimulated Glucose Uptake Involves the Transition of Glucose Transporters to a Caveolae-Rich Fraction within the Plasma Membrane: Implications for Type II Diabetes

Fig. 1

Effects of insulin on GLUT4 subcellular location.

Rat adipocytes were incubated in the presence or absence of 5 nM insulin for 10 min and homogenized. Plasma membrane, caveolae, and microsomal fractions were prepared as described in the Materials and Methods. Equal amounts of samples obtained from insulin-treated and nontreated cells were subjected to SDS-PAGE (i.e., the amount of protein is not comparable between the different membrane preparations) and immunoblotted for GLUT4, caveolin, or Na,K-ATPase α 2 subunit. (Lanes 1 and 2) Plasma membranes. (Lanes 3 and 4) Caveolae. (Lanes 5 and 6) Microsomal fraction. In Lanes 1, 3, and 5, cells were incubated without insulin; in Lanes 2, 4, and 6, with insulin. The positions of molecular mass markers (Rainbow, Amersham) are indicated: ovalbumin, 46 kD; Phosphorylase, 97 kD, trypsin inhibitor, 21 kD.

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Molecular Medicine

ISSN: 1528-3658