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Fig. 6 | Molecular Medicine

Fig. 6

From: Cyclooxygenase-2-Dependent Bronchoconstriction in Perfused Rat Lungs Exposed to Endotoxin

Fig. 6

RT-PCR analysis of lung tissue for cy-clooxygenase-2 (COX-2) and β -actin message

Lungs were pretreated with 0.3 µM actinomycin D (AcD) or 100 µM cycloheximide (Chx) 10 min before injection of LPS (50 µg/ml). After perfusion for 110 min with LPS (or control) alveolar macrophages were removed by lavage and lung tissue was analyzed for COX-2-specific mRNA. Please note that in these experiments lungs from male Sprague-Dawley rats were used.

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