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Fig. 2 | Molecular Medicine

Fig. 2

From: Induction of Antigen-Specific Tumor Immunity by Genetic and Cellular Vaccines against MAGE: Enhanced Tumor Protection by Coexpression of Granulocyte-Macrophage Colony-Stimulating Factor and B7-1

Fig. 2

Immunization against B16 tumor cells expressing MAGE-1 and MAGE-3 by intramuscular injection of plasmid DNA

Mice were immunized by injections of plasmids into both tibialis anterior muscles or subcutaneously with embryonic fibroblasts and challenged subcutaneously with tumor cells 2 weeks after the last immunization. (A) Mice (n = 5/group) were injected twice separated by 2 weeks with 0.9% saline (□; O), 170 µg (85 µg/muscle) of pCMV-MAGE-1 (♦), or 170 µg of pCMV-MAGE-3 () and challenged with 1.5 × 106 B16 tumor cells expressing MAGE-1 (□; ♦) or MAGE-3 (O; ). (B) Mice (n = 8/group) were injected once with 0.9% saline (□), 100 µg (50 µg/muscle) of pCMV-MAGE-1 (♦), 2.3 × 106 control embryonic fibroblasts (O), or 2.3 × 106 embryonic fibroblasts expressing MAGE-1 (). Animals were challenged with 2 × 106 B16-MAGE-1.

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