Expression of PAI-1 mRNA in the tissues of lean and obese mice
Total RNA was extracted from 7- to 8-week-old male lean (□) and obese (■) animals and PAI-1 mRNA was determined using quantitative PCR analysis, n = 6 ± SD. Inset: representative Northern blot showing PAI-1 mRNA expression in adipose tissues from obese (odd-numbered lanes) and lean (even-numbered lanes) mice. Lanes 1 and 2, epididymal fat; Lanes 3 and 4, subcutaneous fat; Lanes 5 and 6, brown fat. 18S rRNA is shown as a control for loading and for the integrity of the RNA.