Fig. 5From: Tissue Distribution and Regulation of Plasminogen Activator Inhibitor-1 in Obese Mice Cellular localization of PAI-1 mRNA and antigen in the adipose tissue from lean (+/?) and obese (ob/ob) mice Vasculature from adipose tissue of lean (A and C) and obese (B and D) mice analyzed for PAI-1 mRNA (A and B)) and antigen (C and D). Sections of epididymal fat pads containing adipocytes and microvascular endothelial cells from lean (E and G) and obese (F and H) mice analyzed for PAI-1 mRNA (E and F) and antigen (G and H). e, endothelial cell; s, smooth muscle cells; tm, tunica media, arrowheads, positive cells. Slides for in situ hybridization were exposed for 8 weeks at 4°C and stained with hematoxylin and eosin. Slides used for immunohistochemical analysis were counterstained with hematoxylin. Magnification: 400 × for all sections.Back to article page