Fig. 6
From: Tissue Distribution and Regulation of Plasminogen Activator Inhibitor-1 in Obese Mice
Effect of insulin on the plasma levels of active PAI-1, glucose, and total insulin in lean mice
Six- to 8-week-old male lean mice were injected intraperitoneally with saline or 10 units regular human insulin. The animals were sacrificed at the indicated times and blood was collected into 20 mM EDTA (final concentration). After centrifugation, the plasma was collected and PAI-1 activity (ng/ml) determined using the t-PA binding assay (A). Glucose levels and total plasma insulin levels were determined using the hexokinase assay and a rat insulin RIA assay, respectively (B).