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Fig. 4 | Molecular Medicine

Fig. 4

From: Aspirin-Triggered Lipoxins (15-epi-LX) Are Generated by the Human Lung Adenocarcinoma Cell Line (A549)–Neutrophil Interactions and Are Potent Inhibitors of Cell Proliferation

Fig. 4

Products from epithelial cell-PMN costimulation

RP-HPLC chromatogram (left panel) and on-line UV spectra (right panels). Confluent A549 cells were exposed to IL-1β (1 ng/ml) for 24 hr, treated with ASA (20 min) and arachidonic acid (20 µM, 60 s), and each incubated with freshly isolated PMN (A549 cell:PMN cell ratio of 1:8) followed by stimulation with ionophore A23187 (5µM) in 4 ml of HBSS for 30 min at 37°C. Products were extracted and taken to RP-HPLC as described in Materials and Methods. The chromatogram was plotted at 300 nm and is representative of n = 6 experiments. Displayed spectra are from material eluting beneath Peak B (identified as predominantly 15-epi-LXB4) and 15-epi-LXA4 (see text for details). Similar spectra, each showing absorbance consistent with tetraene chromophores, were obtained from LXA4 and Peaks A and C.

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