ASA modulates both LX (A) and pLT (B) formation during epithelial cell-PMN costimulation
A549 cells were exposed to IL-1β (1 ng/ml, 24 hr) and treated (20 min, 37°C) with either vehicle (0.1% v/v) or ASA, before the addition of arachidonic acid (20 µM, 1 min) and freshly isolated PMN (A549/PMN cell ratio of 1:5). Costimulations were carried out as in Fig. 4. Results represent the mean ± SEM from three to five separate donors. Insets: Effect of cell ratio on generation of tetraene-containing LXs (LX plus 15-epi-LX) (A) and pLTs (LTC4 plus LTD4) (B) during co-incubations of A549 cells with PMN in the absence (○) or presence (■) of ASA.