Fig. 6
LX inhibits A549 cell proliferation
(A) Time-dependent growth arrest. A549 cells in 96-well plates were treated with either vehicle (0.15% EtOH) or equimolar concentrations (10−6 M) of LXA4, LXB4, or Dex for up to 96 hr at 37°C. At the indicated intervals, cells were harvested for the MTT assay. Data are mean ± SEM of three to seven experiments performed in quadruplicate. *p < 0.05 and **p < 0.005 for compounds versus vehicle. (B) Concentration dependence. A549 cells were exposed to LXA4, LXB4, or Dex at the indicated concentrations for 72 hr at 37°C. Results are mean ± SEM of five to eight experiments performed in quadruplicate. Results are expressed as the percent inhibition of proliferation relative to vehicle. *p < 0.05, **p < 0.025, and ***p < 0.005 for compounds versus vehicle.