(A) Expression of hGRβ in human tissues. hGRβ protein is expressed in multiple human tissues. Protein extracts (25 µg/lane) were run on a 8% SDS Polyacrylamide gel. After transfer to nitrocellulose membranes, samples were probed with anti-hGRβ specific antibody. The positions of the molecular-mass marker expressed in kD are indicated by arrows on the left. (B) Cytosolic and nuclear localization of the hGR isoforms in the presence and absence of dexamethasone. hGRα (left) and hGRβ (right) immunoblotting analyses of nuclear (N, Lanes 1 and 2) and cytosolic (C, Lanes 3 and 4) fractions of HeLa cells. Proteins (8 µg/lane) were run on a 8% SDS Polyacrylamide gel, in the absence (Lanes 1 and 3) or presence (Lanes 2 and 4) of 10−7 M dexamethasone. The positions of the molecular-mass markers expressed in kD are indicated by arrows on the left.