TCR BV gene segment usage in CD4
Five normal individuals and 10 RA patients were identified who had more than 2.5% of CD4+ CD28− T cells (Fig. 1). PBMC were analyzed by three-color FACS analysis with PercP-anti-CD4, PE-anti-CD28, and FITC-anti-BV element. Compared with the CD4+ CD28+ compartment (▨), the TCR β-chain repertoire of CD4+ CD28− T cells (■) was strongly skewed toward the preferential usage of few BV elements. Three representative individuals are shown; results for all normal individuals and RA patients are summarized in Table 1.