Fig. 8
From: A Novel Protease Homolog Differentially Expressed in Breast and Ovarian Cancer
Expression of protease M protein in mammary cell lines and insect cells infected with recombinant protease M baculovirus
Fifty micrograms of total cell lysates from mammary cell lines, uninfected insect cells (SF9), and insect cells infected with either 4.5 µl of recombinant protease M baculovirus (SF9/1G3 [1]) or 22.5 µl of recombinant baculovirus (SF9/IG3 [2]) were run on a 12% polyacryamide/SDS gel, transferred to PDVF membrane, and reacted with protease M polyclonal anti-peptide antibody as the primary antibody and horseradish peroxidase-conjugated anti-rabbit IgG secondary antibody. Bands were detected with ECL.