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Table 1 Expression of protease M mRNA and protein in mammary cells

From: A Novel Protease Homolog Differentially Expressed in Breast and Ovarian Cancer

Cell Line

RNA a

Protein b

70N

  

81N

  

16-1-1 (76N/HPV16)

  

2 INT

  

21PT

  

MDA.MB435

  

T47D

  

MCF-7

  
  1. aRNA values were obtained by running 10 µg of total RNA on a Northern blot, by hybridizing it to 32P-labeled protease M probes, and by quantitating the resulting autoradiograms. The most intense band was set equal to 100 and the other values normalized accordingly.
  2. bProtein values were obtained by running 50 µg of total cell lysates on a Western blot and probing it with the protease M antibody, as described in Materials and Methods. The 37-kD bands on the autoradiograms were quantitated; the most intense band was set equal to 100 and the other values normalized accordingly.