Cell Line
|
RNA
a
|
Protein
b
|
---|
70N | Â | Â |
81N | Â | Â |
16-1-1 (76N/HPV16) | Â | Â |
2 INT | Â | Â |
21PT | Â | Â |
MDA.MB435 | Â | Â |
T47D | Â | Â |
MCF-7 | Â | Â |
- aRNA values were obtained by running 10 µg of total RNA on a Northern blot, by hybridizing it to 32P-labeled protease M probes, and by quantitating the resulting autoradiograms. The most intense band was set equal to 100 and the other values normalized accordingly.
- bProtein values were obtained by running 50 µg of total cell lysates on a Western blot and probing it with the protease M antibody, as described in Materials and Methods. The 37-kD bands on the autoradiograms were quantitated; the most intense band was set equal to 100 and the other values normalized accordingly.