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Table 1 Expression of protease M mRNA and protein in mammary cells

From: A Novel Protease Homolog Differentially Expressed in Breast and Ovarian Cancer

Cell Line RNA a Protein b
70N   
81N   
16-1-1 (76N/HPV16)   
2 INT   
21PT   
MDA.MB435   
T47D   
MCF-7   
  1. aRNA values were obtained by running 10 µg of total RNA on a Northern blot, by hybridizing it to 32P-labeled protease M probes, and by quantitating the resulting autoradiograms. The most intense band was set equal to 100 and the other values normalized accordingly.
  2. bProtein values were obtained by running 50 µg of total cell lysates on a Western blot and probing it with the protease M antibody, as described in Materials and Methods. The 37-kD bands on the autoradiograms were quantitated; the most intense band was set equal to 100 and the other values normalized accordingly.