Fig. 5
From: Molecular Cloning of the Human Leukotriene C4 Synthase Gene and Assignment to Chromosome 5q35
Fluorescent in situ hybridization
Metaphase chromosomes were hybridized with purified DNA from the P1 clone labeled with digoxigenin dUTP by nick translation. Specific hybridization signals were detected by incubating with a fluoresceinated anti-digoxigenin antibodies. Doublet positive signals are observed on the far distal end of the long arm (q arm) of a group B chromosome (distal arrow). Measurements of 10 chromosomes revealed that the signal was localized 99% of the distance from centromere to the telomere. Metaphase chromosomes were also hybridized with a control probe that is known to localize to 5q21 (proximal arrow). These data taken together indicate that LTC4 synthase localizes to 5q in an area corresponding to band 35 (5q35).