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Fig. 5 | Molecular Medicine

Fig. 5

From: Molecular Cloning of the Human Leukotriene C4 Synthase Gene and Assignment to Chromosome 5q35

Fig. 5

Fluorescent in situ hybridization

Metaphase chromosomes were hybridized with purified DNA from the P1 clone labeled with digoxigenin dUTP by nick translation. Specific hybridization signals were detected by incubating with a fluoresceinated anti-digoxigenin antibodies. Doublet positive signals are observed on the far distal end of the long arm (q arm) of a group B chromosome (distal arrow). Measurements of 10 chromosomes revealed that the signal was localized 99% of the distance from centromere to the telomere. Metaphase chromosomes were also hybridized with a control probe that is known to localize to 5q21 (proximal arrow). These data taken together indicate that LTC4 synthase localizes to 5q in an area corresponding to band 35 (5q35).

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