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Fig. 2 | Molecular Medicine

Fig. 2

From: The Alzheimer’s Disease-Associated Presenilins Are Differentially Phosphorylated Proteins Located Predominantly within the Endoplasmic Reticulum

Fig. 2

Subcellular localization of endogenous PS proteins in CHO cells analyzed by confocal microscopy

Endogenous PS proteins expressed in CHO cells (PS) were detected with the affinity-purified antibody TOR519 (A, PS). Double labeling with antibody TOR519 (A, PS) and a monoclonal antibody to grp78 (BiP) revealed a highly similar staining (B, ER). Superimposing the images obtained in Panels A and B reveals an almost complete overlapping staining pattern (C, ER & PS) indicating that endogenous PS proteins are located within the ER. (D and E) CHO cells were stained with the affinity purified antibody TOR519 (D) or with TOR519 preabsorbed with the corresponding peptide antigen and analyzed by light microscopy (E). Preabsorption of TOR519 clearly inhibits the staining pattern observed in Panel D. (F) CHO cells stained with the secondary antibody alone. Note, that PS proteins were detected within the nuclear envelope but not within the nucleus (compare the confocal image in Panel A and the light microscopy photograph in Panel D).

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