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Fig. 4 | Molecular Medicine

Fig. 4

From: The Alzheimer’s Disease-Associated Presenilins Are Differentially Phosphorylated Proteins Located Predominantly within the Endoplasmic Reticulum

Fig. 4

Cellular localization of full-length PS-2 (A) and the C-terminal truncated derivative ending after TM4 (F) in COS-7 cells transfected with the corresponding cDNAs

Cells were double immunostained using the affinity purified antibody TOR519 (B and G) and with antibodies against grp78 (BiP; C and H) to detect the ER or with WGA to detect the Golgi compartment (50) (D, E, I, and K). No major differences were observed in the cellular localization of truncated PS-2 TM4 proteins as compared to full-length PS-2. Wt and recombinant PS-2 proteins are predominantly located within ER and to a smaller extent in the Golgi, as shown above (Fig. 3). Note, that cells transfected with the deletion construct did not react with antibody BOS4627 (data not shown), which clearly shows the specificity of the antibodies used. Moreover, antibody TOR519 strongly detects a few transfected cells while the majority of the (untransfected) cells is stained only very weakly, again strongly supporting the antibody specificity. The Golgi is marked by arrowheads in Panels D and E as well as in Panels I and K.

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