Fig. 7
Stimulation or inhibition of PKC has no influence on PS-2 phosphorylation
COS-7 cells transfected with the PS-2 cDNA were treated with either DMSO alone (control), with PDBu (to stimulate PKC), or with GF109203X, a specific inhibitor of PKC, in the presence of [32P]-orthophosphate. Cell lysates were immunoprecipitated with antibody BOS4627. The ∼100-kD protein (*) is also detected in mock-transfected cells. Arrowheads indicate the putative PS monomers and dimers. Oligomers of higher molecular weight were revealed after longer exposure.