Fig. 1From: Elevated Expression of Transforming Growth Factor-β in Adipose Tissue from Obese MiceExpression of TGF-ß mRNA in adipose tissues from lean and obese mice.(A) Total RNA was extracted from epididymal fat pads of male lean and obese (ob/ob; db/db) animals of the indicated ages. TGF-β mRNA was determined using quantitative PCR analysis, n = 6 ± SD. (B) Cell fractionation of adipose tissue from lean and obese mice. Epididymal fat pads were isolated from 6-month-old male lean and ob/ob mice. Adipocytes (AD) and stromal vascular cells (S/V) were separated by collagenase digestion followed by differential centrifugation. Total RNA was extracted from each fraction and TGF-β mRNA levels were quantified by RT-PCR. n = 3.Back to article page