Fig. 4
From: Elevated Expression of Transforming Growth Factor-β in Adipose Tissue from Obese Mice
Plasma PAI-1 activity and PAI-1 mRNA expression in cell-fractionated adipose tissue after TGF-β treatment.
(A) Six- to eight-week-old male lean CB6 mice were injected intraperitoneally with 2 µg human recombinant TGF-β or diluent. The animals were sacrificed 3 hr later and blood was collected into 20 mM EDTA (final concentration). After centrifugation, the plasma was collected and active PAI-1 (ng/ml) was determined using the t-PA binding assay. (B) Epididymal fat pads were isolated from 6- to 8-week-old CB6 mice 3 hr after intraperitoneal injection of TGF-β (2 µg). Mature adipocytes (AD) and stromal vascular cells (S/V) were separated by differential centrifugation and total RNA was prepared and PAI-1 mRNA levels were determined using RT-PCR. n = 3, ± SD.