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Fig. 7 | Molecular Medicine

Fig. 7

From: mMaspin: The Mouse Homolog of a Human Tumor Suppressor Gene Inhibits Mammary Tumor Invasion and Motility

Fig. 7

The expression and purification of mMaspin revealed by SDS-PAGE followed by standard Coormassie Briliant Blue R-250 staining

Each lane (except lane 5) contains 20 µg of the total proteins extracted from bacteria XL1-blue transformed by pGST-mMaspin in lysis buffer containing 1% Triton X-100. Cells were grown for 1–2 hr with or without IPTG (0.2 mM). The GST-mMaspin fusion protein has a size of 67 kd. Lane 5, 1 µg of affinity purified GST-mMaspin.

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