T Cell Subset | Stimuli | Times Increase per T Cell Subset after Culture |
|---|
HIV− Donorsa | HIV+ Donors |
|---|
SN1 | SN2 | SN3 | SN4 | SN5 | SP1 | SP2 | SP3 | SP4 | SP5 | SP6 | SP7 |
|---|
TCR γδ | Daudi | 4.0 | 6.2 | 5.6 | 4.9 | 3.2 | 0 | 0 | 1.6 | 0 | 0 | 0 | 0 |
TCR γδ | Etpp | 17 | 22 | nt | nt | nt | 0 | 0 | 1.5 | 0 | nt | nt | nt |
TCR αβ | Daudi | 0 | 0 | 1.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
TCR αβ | Etpp | 0 | 0 | nt | nt | nt | 0 | 0 | 0 | 0 | nt | nt | nt |
- PBMCs from each donor were cultured in the presence of either irradiated Daudi cells, or Etpp and IL-2 (to achieve a maximal response) as described in Materials and Methods. After 8 days, the resulting times increase over the input number of the particular lymphocyte population was measured. The input numbers of αβ/γδ T cells (in millions) for each set of cultures were as follows: SN1, 1.32/0.053; SN2, 0.90/0.042; SN3, 0.49/0.035; SN4, 0.72/0.010; SN5, 0.38/0.053; SP1, 0.87/0.072; SP2, 0.76/0.020; SP3, 1.14/0.80; SP4, 0.28/0.006; SP5, 0.42/0.043; SP6, 0.72/0.043; SP7, 0.34/0.009. 0 = no increase above the input number; nt = not tested.
- aIn a separate experiment, Vγ9/Vδ2 T cells from 32 out of 32 putatively healthy blood donors responded positively to a different batch of Daudi cells (see Discussion). Since different batches and passages of Daudi cells differ slightly in their stimulatory activities for Vγ9/Vδ2 T cells, this experiment has qualitative rather than quantitative significance in the context of present data.
