Fig. 5

Expression of CD3-ε derived transgenes induces transition from DN to DP thymocytes in young RAG-2^null mice.

Thymocytes were analyzed by three-color staining with combinations of antibodies against Thy-1, CD44, and CD25; and CD4, CD8, and TCR-αβ. Thymocytes were derived from a young RAG-2^null × tgεΔ1-2966H^+/− mouse, from a RAG-2^null mouse 1 month after i.p injection of an anti-CD3-ε antibody, and from a RAG-2^null control mouse. Most of the thymocytes (>90%) from all of these mice were Thy-l⁺ and TCR-αβ⁻ (not shown).