Fig. 6
Enrichment of γδ T cells among peripheral blood (PB) and colonic mucosal (ML) T cells by adherence to fibroblast cell lines
(A) Selective adherence of γδ T cells to fibroblast cell lines of intestinal origin. T cells isolated from the blood of normal healthy donors (N-PB) and CD patients (CD-PB) and from segments of inflamed (involved) colon from CD patients (CD-ML) were co-cultured with different established fibroblast cell lines for 30 min at 37°C. The fold-enrichment of γδ T cells among the adherent cells was determined by comparing the number of γδ T cells in the input and adherent fraction, the values for which were obtained by staining with anti-TCR antibodies and flow cytometry. (B) Preferential adherence of mucosal γδ T cells to colonic fibroblasts. Colonic T cells isolated from segments of involved (I) and noninvolved (N) colon from the same CD patient were cultured with the colonic fibroblast cell line, CCD-18Co, for 30 min. Fold-enrichment of adherent αβ and γδ T cells was determined by flow cytometry.