Fig. 3

Reduction in A β production and release after PDBu

(A) Representative autoradiograms after the two different labeling methods. In both approaches, PDBu was added to wild-type CHO cells during the 2-hr chase period after either a 10-min pulse labeling by ³⁵S-methionine or ¹²⁵I surface iodination. The decrease in Aβ release in medium from ³⁵S-methionine-labeled cells is mirrored by an increase in p3 fragment. Recovery of p3 after surface iodination is inconsistent, as was shown previously (6). (B) The average percent reduction (± SEM) of Aβ release after ³⁵S-methionine (n = 4) and ¹²⁵I surface labeling (n = 3). The difference between the two labeling methods was statistically significant (p < .005).