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Fig. 3 | Molecular Medicine

Fig. 3

From: Genetically Transferred Central and Peripheral Immune Tolerance via Retroviral-Mediated Expression of Immunogenic Epitopes in Hematopoietic Progenitors or Peripheral B Lymphocytes

Fig. 3

Induction of peptide-specific humoral immune tolerance in adult bone marrow chimeras infused with peptide-Ig-expressing progenitor cells

BALB/c mice were sublethally irradiated with either (A) 200 rads, or (B) 600 rads and infused with 1−2 × 106 gene-transferred (+, triangles) or mock-transduced (−, circles) BM cells. Mice were primed and boosted for humoral responses either (A) 1 month or (B) 2 months post-infusion with synthetic 12–26 peptide, and with HEL as a specificity control. Nonmanipulated, immunized BALB/c always produced titers similar to recipients infused with mock-transduced BM cells (A, diamonds). Both total peptide-specific IgG (open symbols), or the main isotype IgG1 (closed symbols) were diminished in all experiments (both shown only for A). Normal BALB/c recipients in (B) received either 5-FU-pretreated normal BALB/c BM or SCID/BALB/c BM cells. Flow cytometry analysis at sacrifice time in (B) revealed comparable levels of CD4+ and Ig+ splenocytes in normal BALB/c reconstituted with either normal or SCID BM: (CD4+: 18–25%; Ig+: 40–65%). All experiments have been done at least twice with 3–5 mice per group with similar results.

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