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Fig. 5 | Molecular Medicine

Fig. 5

From: Priming of Cytotoxic T Lymphocytes by DNA Vaccines: Requirement for Professional Antigen Presenting Cells and Evidence for Antigen Transfer from Myocytes

Fig. 5

NP-specific CTL responses induced by i.m. NP DNA immunization are restricted by the BM MHC haplotype in H-2 d×k BM chimeric mice

H-2d → H-2d×k (A) and H-2k → H-2d×k (B) chimeras were injected i.m. with NP DNA (100 µg) on Weeks 0, 3, and 6 and spleen cells were prepared on Week 8. Cells were restimulated with F1 spleen cells infected with A/PR/8/34 influenza virus for 7 days. P815 (H-2d) and L929 (H-2k) target cells were infected with influenza A/Victoria/73 virus (solid bars), or pulsed with the H-2Kk peptide NP 50–57 (shaded bars) or H-2Kd peptide NP 147–155 (striped bars) and incubated with CTL at an E:T ratio of 50:1. Data are plotted as percent specific lysis ± SD, where n = 3.

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