Fig. 6

Induction of CTL in H-2 ^d×k BM chimeric mice after transplantation of NP-expressing myoblasts

H-2^d → H-2^d×k mice were injected i.m. with NP-expressing myoblasts and spleen cells were prepared 6 weeks after transplantation. Cells were restimulated with F1 spleen cells pulsed with H-2K^k peptide NP 50–57 or H-2K^d peptide NP 147–155 for 7 days. P815 (H-2^d) and L929 (H-2^k) target cells were infected with influenza A/Victoria/73 virus (solid bars), or pulsed with the H-2K^k (shaded bars) or H-2K^d (striped bars) peptides and incubated with CTL at an E:T ratio of 50:1. Data are plotted as percent specific lysis ± SD, where n = 3. Similar results were seen when restimulation was performed by using influenza virus-infected F1 spleen cells as stimulators (results not shown).