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Table 1 Absence of effect of superoxide on glutamate uptake

From: Inhibition of Astrocyte Glutamate Uptake by Reactive Oxygen Species: Role of Antioxidant Enzymes

Condition

Glutamate Uptake (% of Control)

H2O2

46 ± 2**

H2O2 + catalase

100 ± 3

Xanthine + xanthine oxidase

48 ± 1**

Xanthine + xanthine oxidase + catalase

101 ± 3

Xanthine + xanthine oxidase + SOD

46 ± 1**

Potassium superoxide

103 ± 2

  1. Reagents were applied at the following concentrations: H2O2, 2 mM; xanthine, 1 mM; xanthine oxidase, 200 mU/ml; catalase, 800 U/ml; SOD, 100 U/ml; potassium superoxide, 1 mM. H2O2, the mixture containing xanthine and xanthine oxidase, and potassium superoxide were added 10 min before incubation with 3H glutamate. **p < 0.01.
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Molecular Medicine

ISSN: 1528-3658