Skip to main content
Fig. 4 | Molecular Medicine

Fig. 4

From: Multiple Mechanisms Support Oligoclonal T Cell Expansion in Rheumatoid Synovitis

Fig. 4

Identification and characterization of CD4 + T cell clones expanded in the peripheral blood and the synovial tissue

The CD4+ IL-2R+ and CD4+ IL-2R subsets were analyzed as outlined in Fig. 1. The BV5S1-BJ2S1 amplification products from peripheral blood and synovial compartments of metacarpal joints (MCP) of patient EK2 are shown. All compartments contained a dominant band of equal size (a) which represented the identical clonotype as determined by direct sequencing (BV5S1 CASSLDARNEQFF BJ2S1). Sequences of ubiquitous clones from 8 patients out of a cohort of 14 patients are shown in Table 4. These clones are generally found in the IL-2R+ as well as the IL-2R subset, suggesting continuous stimulation. In addition to this ubiquitous T cell clone, a T cell clone restricted to the IL-2R+ subset (b) of synovial CD4+ T cells was identified (BV5S1 CASSLDARNEQFF BJ2S1).

Back to article page