Skip to main content
Fig. 1 | Molecular Medicine

Fig. 1

From: Versatile Retrovirus Vector Systems for Regulated Gene Expression In Vitro and In Vivo

Fig. 1

Schematic map of SFG-based retroviral vectors used in the study

Vectors employed in the“two virus”strategy (A) and“single virus”strategy (B) are shown. For the“two virus”strategy, the vectors include SFG vectors used for constitutive gene expression of the transactivators tTA or rtTA (depicted as SFG tTA), and (i) a retroviral vector in which tetOP/minimal CMV promoter sequences are introduced internal to the pro-viral transcriptional unit (SFG tc LucECT), (ii) retroviral vectors in which tetracycline response elements (tetOP) are introduced into a 3′LTR in which the enhancer (or CAAT sequences) are deleted (depicted as SFG Luct7E) (the vector carrying a deletion of enhancer and CAAT sequences, termedSFG Luc7EC, is not shown), or (iii) a retroviral vector in which the tetOP/minimal CMV promoter sequences are introduced in the 3′LTR in place of the viral enhancer/promoter sequences (SFG Luc t7CM). Also shown is a vector representing the“single virus”strategy (SFG Luc tc LucIT). For all constructs, tTA or rtTA (shaded boxes) represent the original or modified transactivator ORFs; tet (black boxes) represent the tandem repeats of tet operator sequences; CMV (lightly shaded boxes) represents the CMV IE minimal promoter; Luc (open boxes) represents the luciferase ORF; IRES (striped boxes) represents the EMCV internal ribosomal entry site; SD, splice donor; SA, splice acceptor; LTR, long terminal repeat. Details of the construction of the different retroviral vectors are provided in the Materials and Methods section.

Back to article page