Fig. 1

Age-dependent increase of NF-κB activity in the rat liver

The picture shows results of electrophoretic mobility shift assay of liver nuclear extracts derived from duplicate animals of 3, 18, and 22 months of age. A DNA duplex spanning −574 to −554 base pair positions of the rat androgen receptor gene promoter containing the NF-κB binding site was used as the radiolabeled probe. (These experiments were performed by Dr. P.C. Supakar in our laboratory.)