Fig. 2
Ad5.rsIL-6R cell infection gives rise to specific mRNA for rat sIL-6R
Total RNA from different recombinant adenovirusinfected HepG2 or H35 cells were analyzed by Northern blot and probed with an sIL-6R-specific 32P-labeled oligonucleotide. The upper panel shows the specific hybridization only with RNA extracted from Ad5.rsIL-6R-infected cells (lanes R), whereas Ad5.E1.LacZ (lanes Z) or Ad5.IL-6 (vector expressing rat IL-6) (lanes 6) do not give any signal. The bottom panel is the gel stained with ethidium bromide.