Cytokine expression in fresh temporal artery specimens from GCA patients and in specimens explanted after 4 weeks
Biopsy specimens from eight GCA patients were cut into small fragments and either shock frozen or implanted into C.B.-17 SCID mice. Paired samples were analyzed in parallel. Total RNA was extracted, cDNA was adjusted for the number of β-actin copies, and cytokine sequences were semiquantified by PCR and subsequent oligonucleotide hybridization. Results are expressed as number of copies per 200,000 β-actin copies. (A) T cell-derived cytokines; (B) macrophage-derived cytokines.