Table 1 14 C-inulin permeability and monocyte transmigration across a brain microvascular endothelial barrier cultured on various extracellular matrix a
From: TNF-α Opens a Paracellular Route for HIV-1 Invasion across the Blood-Brain Barrier
Extracellular Matrix | Coefficient of Permeability to 14C-inulin (cm/min)b | No. of Transmigrated Monocytesb |
|---|---|---|
None | 0.0032 ± 0.0001 | >10,000 |
Collagen I-rat tail | 0.0010 ± 0.0000 | 634 ± 64 |
Collagen IV-mouse | 0.0009 ± 0.0001 | N.D. |
Collagen I-fibronectin | 0.0009 ± 0.0001 | N.D. |
Human fibronectin | 0.0023 ± 0.0009 | 1758 ± 252 |
Mouse laminin | 0.0017 ± 0.0008 | N.D. |
MATRIGEL | 0.0003 ± 0.0000 | 0 ± 0 |
p ]a40,000 brain endothelial cells were seeded into inserts with a terephthalate membrane bottom coated with specified matrix and cultured for 3 days. bMean ± S.D.M.; N.D., not determined. | ||