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Table 1 14 C-inulin permeability and monocyte transmigration across a brain microvascular endothelial barrier cultured on various extracellular matrix a

From: TNF-α Opens a Paracellular Route for HIV-1 Invasion across the Blood-Brain Barrier

Extracellular Matrix Coefficient of Permeability to 14C-inulin (cm/min)b No. of Transmigrated Monocytesb
None 0.0032 ± 0.0001 >10,000
Collagen I-rat tail 0.0010 ± 0.0000 634 ± 64
Collagen IV-mouse 0.0009 ± 0.0001 N.D.
Collagen I-fibronectin 0.0009 ± 0.0001 N.D.
Human fibronectin 0.0023 ± 0.0009 1758 ± 252
Mouse laminin 0.0017 ± 0.0008 N.D.
MATRIGEL 0.0003 ± 0.0000 0 ± 0
p ]a40,000 brain endothelial cells were seeded into inserts with a terephthalate membrane bottom coated with specified matrix and cultured for 3 days. bMean ± S.D.M.; N.D., not determined.