Analysis of total RNA from esophagus
Five micrograms of total RNA from esophagus tissue samples was analyzed using the same conditions, antisense probe combinations, and experimental groups as used for comb analysis, with the exception that 33-day-old (33d) UCD-200 and UCD-058 chickens were also examined. (A) RPA blot: Arrows indicate the two mRNA variants of α2(I) procollagen. (B) Quantification: For statistical analysis of procollagen mRNA patterns in internal organs, material from 7- to 11-day-old (7-11d) UCD-200 chickens exhibiting different stages of comb involvement was pooled and compared with UCD-058 controls. Tissue samples from 33-day-old (33d) and 8-month-old (8m) UCD-200 and UCD-058 chickens were also examined. No statistically significant differences were found between diseased chickens and healthy controls of the same age.