Altered Procollagen mRNA Expression during the Progression of Avian Scleroderma

Table 1 Subcloning of procollagen cDNA fragments

cDNA	Restriction Sites Used for Subcloning	Length of Subcloned Fragment
α₁(I) pCAL1	PstI-HpaII	250 bp
α₂(I) pCAL3	BamHI-EcoRI	180 bp
α₁(II) pCAR1	PstI-BamHI	310 bp
α₁(III) pIN3	EcoRI-BamHI	190 bp
α₁(VI)	PstI-BamHI	410 bp
α₂(VI) 4aU	EcoRI-PstI	270 bp
α₃(VI)	SacI-HindIII	370 bp
GAPDH pGAD28	HindIII-PstI	150 bp

For in vitro transcription, cDNA fragments were subcloned into the bacterial plasmid vector pGEM4Z by using the above listed restriction enzymes.

ISSN: 1528-3658