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Fig. 7 | Molecular Medicine

Fig. 7

From: Characterization of New Polyclonal Antibodies Specific for 40 and 42 Amino Acid-Long Amyloid β Peptides: Their Use to Examine the Cell Biology of Presenilins and the Immunohistochemistry of Sporadic Alzheimer’s Disease and Cerebral Amyloid Angiopathy Cases

Fig. 7

ELISA assays

(A and B) 750 pg of Aβ40 or Aβ42 (in 25 µl) was mixed with paramagnetic beads (25 µl), electrochemiluminescent-labeled (ECL) FCA3340 or FCA3542 (25 µl, 1 µg/ml), and biotinylated-6E10 (A) or 4G8 (B) in the conditions described in Materials and Methods. After 2 hr, samples were read using an IGEN Origen analyser. Bars are the means ± SEM of independent determinations. Standard curves for Aβ40 (C) and Aβ42 (D) were obtained with various concentrations of synthetic Aβs (in 25 µl), ECL-FCA3340 (filled circles) or ECL-FCA3542 (open circles) (25 µL 1 µg/ml), biotinylated 4G8 (25 µL) and paramagnetic beads (25 µL). Signals were monitored as described above.

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