Fig. 7From: Characterization of New Polyclonal Antibodies Specific for 40 and 42 Amino Acid-Long Amyloid β Peptides: Their Use to Examine the Cell Biology of Presenilins and the Immunohistochemistry of Sporadic Alzheimer’s Disease and Cerebral Amyloid Angiopathy Cases ELISA assays (A and B) 750 pg of Aβ40 or Aβ42 (in 25 µl) was mixed with paramagnetic beads (25 µl), electrochemiluminescent-labeled (ECL) FCA3340 or FCA3542 (25 µl, 1 µg/ml), and biotinylated-6E10 (A) or 4G8 (B) in the conditions described in Materials and Methods. After 2 hr, samples were read using an IGEN Origen analyser. Bars are the means ± SEM of independent determinations. Standard curves for Aβ40 (C) and Aβ42 (D) were obtained with various concentrations of synthetic Aβs (in 25 µl), ECL-FCA3340 (filled circles) or ECL-FCA3542 (open circles) (25 µL 1 µg/ml), biotinylated 4G8 (25 µL) and paramagnetic beads (25 µL). Signals were monitored as described above.Back to article page