Fig. 2
From: Detection and Differentiation of the Six Brucella Species by Polymerase Chain Reaction

Amplification of the six Brucella species
The amplification results of the six Brucella species using a set of four primers are shown. The PCR was achieved in 35 cycles, the annealing temperature was 66°C, and the extension time was 90 sec. Lane M, molecular size standard (pBR322/AluI).