Genomic organization of mouse INS elements
(A) Schematic representation of the cloned INS elements (modified from ref. 1). Primers #1 and #2 amplify a 281 nucleotide region of CORE which was used as the probe in panels B and C. (B, C) Mouse genomic DNA isolated from the liver of BALB/c mice (B) or from P3X63Ag8.653 lymphoid cells (C) was digested with the indicated restriction endonucleases and hybridized to a 32P-labeled CORE-specific DNA probe. The positions of the molecular weight markers are shown on the right. (D, E) Genomic DNA isolated from spleen (lane 1) and liver (lane 2) of BALB/c mice, liver of C3H mice (lane 3), or mouse cell lines P3X63Ag8.653 (lane 4), 70Z/3 (lane 5), 3T3 (lane 6), SV-T2 (lane 7), M-MSV (lane 8), BNL SV A.8 (lane 9), LA-4 (lane 10), and RAG (lane 11) was digested with EcoR5 (D) or Rsa1 (E) and hybridized to a 32P-labeled DNA probe shown in panel A. Minor differences in the strength of hybridization signals are due to slight variations in the amount of DNA loaded. No additional differences between lanes was observed in any part of the gel upon longer exposure. Positions of molecular weight markers are shown on the right.