Prevention of NO-induced chromatin degradation by caspase inhibition
CGC were preincubated for 30 min with 2µM MK801, 100 µM z-D-cbk, or solvent control only. Then they were challenged with 150 µM GSNO (GSNO + MK801, GSNO + z-D-cbk, GSNO) or they were left unchallenged (z-D-cbk, control). After 4 hr incubations were stopped and DNA fragmentation was analyzed by field-inversion gel electrophoresis. Less than 5% of CGC had lost membrane permeability at that time point.