Fig. 9
From: Expression of TNF and TNF Receptors (p55 and p75) in the Rat Brain after Focal Cerebral Ischemia
Triple staining (double-fluorescent immunohistochemistry for TNF and NF200 plus TUNEL staining for detection of apoptotic cell death) in ischemic cortex at 24 hr after the MCA occlusion
This triple staining showed that only neurons exhibiting apoptotic changes express TNF IR. Double exposure (A) of green fluorescence for NF200 and bright-field microscopy for TUNEL staining (brown nuclei) resulted in yellowish cytoplasm in TUNEL-positive neurons; alive neurons had green cytoplasm. Only neurons that were TUNEL-positive expressed TNF IR (B, red filter).