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Table 1 β-galactosidase activity g) of COS-7 cells transfected with pCMVβ (20, 40, 80, and 160 µg/ml) and histone H2A (0.03125, 0.0625, 0.125, 0.25, 0.5, 1, 2, and 4 mg/ml)

From: Histone H2A Significantly Enhances In Vitro DNA Transfection

Histone H2A mg/ml (µM) DNA 160 µg/ml (0.0337/µM) DNA 80 µg/ml (0.0168 µM) DNA 40 µg/ml (0.0084, µM) DNA 20, µg/ml (0.0042, µM) 0
4.0 (286.5) 7.80 ± −0.60 4.94 ± 0.35 2.69 ± 2.01 0.55 ± 0.028  
2.0 (143.3) 9.54 ± 1.17 7.92 ± 3.38 0.19 ± 0.14 0.074 ± 0.073  
1.0 (71.6) 0.93 ± 0.17 158.23 ± 18.65 4.70 ± 2.80 −0.23 ± 0.022  
0.5 (35.8) 2.03 ± 0.45 0.93 ± 0.10 195.18 ± 8.77 37.50 ± 1.87  
0.25 (17.9) 0.37 ± 0.10 0.65 ± 0.20 −0.56 ± 0.17 10.80 ± 3.13  
0.125 (8.95) 0.73 ± 0.23 0.88 ± 0.50 −0.62 ± 0.06 1.22 ± 1.37  
0.0625 (4.48) 0.65 ± 0.22 0.98 ± 0.38 −0.93 ± 0.23 −0.59 ± 0.27  
0.03125 (2.24) 1.04 ± 0.47 0.81 ± 0.18 −1.01 ± 0.23 −1.18 ± 0.12  
0 −0.11 ± 0 0.42 ± 0.50 −0.09 ± 0.06 0.65 ± 0.17 0.003 ± 2
  1. The combinations giving the most efficient transfection are shown in bold. The values shown are the means of triplicates ± 1 S.E.