Fig. 3
From: Gene Transfer into Hepatocytes Mediated by Helper Virus-Free HSV/AAV Hybrid Vectors
Photomicrograph showing HSV-1 amplicon vector-transduced fibroblasts and hepatic cells
Helper virus-free stocks (2 µl) of either (A–D) pHSVlac or (E–H) pmalbe/placa were used to infect cultures of (A and E) baby hamster kidney cells (BHK21), (B and F) human lung fibroblasts (Hs913T), (C and G) G6Pase−/− mouse hepatocytes, and (D and H) human hepatocytes (NPLC). Twenty-four hours after infection, cultures were stained with X-Gal. Scale bar, 200 µm.