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Fig. 4 | Molecular Medicine

Fig. 4

From: Gene Transfer into Hepatocytes Mediated by Helper Virus-Free HSV/AAV Hybrid Vectors

Fig. 4

HSV-1 amplicon and HSV/AAV hybrid vector-mediated gene transfer into the mouse liver

Stocks (100 µl, 2 × 107 t.u.) of (A, B) pHSvGN or (C–F) pHyRGN were injected into the left liver lobe of (A, B) RAG2−/− or (C–F) normal mice (C57BL/6). Animals were killed (A, C–E) 1 day or (B, F) 7 days following gene transfer. (G) Mice preinjected with pHyRGN 4 weeks earlier and (H) naive animals were injected intraparenchymally with 1 × 107 t.u. (100 µl) of pHSVlac and killed 1 day later. Liver sections were prepared (20 µm) and (A–F) examined for green-fluorescent hepatocytes using a standard fluorescence microscope or (G and H) histochemically stained with X-Gal. Scale bars: 80 µm (A, B), 100 µm (C–H).

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