Fig. 1

CDR3 length analysis of T cells isolated from peripheral blood (PB) (A, B) and lymph nodes (LN) (C, D) of breast cancer patients

Mononuclear cells were isolated from PB or LN and CD4⁺ (A,C) and CD8⁺ (B,D) cells were positively selected by magnetic beads (see Materials and Methods). CDR3 lengths were analyzed by using a two-step PCR technique with ³²P-labeled C, β reverse primer.