Oligoclonality of CD8+ T Cells in Breast Cancer Patients

Table 1 Sequence of primers used

Primers		Sequence (5′–3′)
First-step PCR		Nested primers
BV1	CAACAGTTCCCTGACTTGCAC	CTCAGCTTTGTATTTCTGTG
BV2	TCAACCATGCAAGCCTGACCT	CAGCAGCCTCTACATCTGCA
BV3	TCTAGAGAGAAGAAGGAGCGC	GACATCTATGTACCTCTGTG
BV4	CATATGAGAGTGGATTTGTCATT	CAGCAGCATATATCTCTGCA
BV5	CCTAACTATAGCTCTGAGCTG	CTCGGCCCTCTATCTCTGTG
BV6	AGGCCTGAGGGATCCGTCTC	CTC(G or C or A)GCC(G or A)TGTATCTCTGTG
BV7	CTGAATGCCCCAACAGCTCTC	CTC(G or A)GCCCTGTATCTCTGCG
BV8	TACTTTAACAACAACGTTCCG	CTCAGCTGTGTACTTCTGTG
BV9	AAATCTCCAGACAAAGCTCAC	CTCTGCTGTGTATTTCTGTG
BV10	CAAAAACTCATCCTGTACCTT	CACAGCACTGTATTTCTGTG
BV11	ACAGTCTCCAGAATAAGGACG	TACCTCTCAGTACCTCTGTG
BV12	GACAAAGGAGAAGTCTCAGAT	GACATCTGTGTACTTCTGTG
BV13.1	GACCAAGGAGAAGTCCCCAAT	GACATCTGTGTACTTCTGTG
BV13.2	GTTGGTGAGGGTACAACTGCC	GACATCTGTGTACTTCTGTG
BV14	TCTCGAAAAGAGAAGAGGAAT	GACCTCTCTGTACTTCTGTG
BV15	GTCTCTCGACAGGCACAGGCT	GACAGCTCTTTACTTCTGTG
BV16	GAGTCTAAACAGGATGAGTCC	TTCTGGAGTTTATTTCTGTG
BV17	CACAGATAGTAAATGACTTTCAG	GACAGCTTTCTATCTCTGTG
BV18	GAGTCAGGAATGCCAAAGGAA	TTCGGCAG(G or C)TTATTTCTGTG
BV19	CCCCAAGAACGCACCCTGC	CACGGCACTGTATCTCTGCG
BV20	TCTGAGGTGCCCCAGAATCTC	CTCTGGCTTCTATCTCTGTG
CB-14	CTCAGCTCCACGTG
CB-R	CTTCTGATGGCTCAAACAC
BJ primer used for sequencing
BJ1S1	AACTGTGAGTCT GGTGCCTT
BJ1S2	ACGGTTAACCTG GTCCCCGA
BJ1S3	CTCTACAACAGT GAGCCAAC
BJ1S4	GACAGAGAGCTG GGTTCCAC
BJ1S5	TGGAGAGTCGAG TCCCATCA
BJ1S6	TGAGCCTGGTCC CATTCCCA
BJ2S1	CCTCTAGCACGG TGAGCCGT
BJ2S2	TACGGTCAGCCT AGAGCCTT
BJ2S3	CTGTCAGCCGGG TGCCTGGG
BJ2S4	CTGAGAGCCGGG TCCCGGCG
BJ2S5	CCTCGAGCACCA GGAGCCGC
BJ2S6	CCTGCTGCCGGC CCCGAAAG
BJ2S7	TGACCGTGAGCC TGGTGCCC

BV: TCR Vβ; BJ: TCR Jβ; CB: TCR Cβ.
CB-14 was used for first-strand cDNA synthesis, and CB-R was used for sequencing.

ISSN: 1528-3658