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Fig. 2 | Molecular Medicine

Fig. 2

From: Transcription Abnormalities Potentiate Apoptosis of Normal Human Fibroblasts

Fig. 2

p53 induction and apoptosis in DNA repair-deficient fibroblasts

Cells were treated with 10 µM mitomycin C and samples were collected at the indicated time points. (A) Extracts of XPG fibroblasts were analyzed for p53 and p21WAF1,Cip1 protein levels, apoptosis-connected proteolytic cleavage of a 42-kDa protein, and for the presence of extractable DNA, as described for normal fibroblasts in Figure 1. (B) Kinetics of p53 induction and apoptosis in mitomycin C-treated primary fibroblasts. Cell samples were analyzed as in A, but only the p53 immunoblots are shown. Arrows indicate the time when first signs of apoptosis were detected, as in Figure 1. M, migration of the 1-kb ladder.

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