Fig. 2
From: Lipopolysaccharide-Induced Cytokine Cascade and Lethality in LTα/TNFα-Deficient Mice
Cytokine production by elicited macrophages stimulated in vitro by LPS
Thioglycollate-elicited macrophages (5 × 105 cells in 0.5 ml of culture medium) from the three genotypes (same symbols as in Fig. 1) were stimulated with the indicated amount of purified Escherichia coli 0111:B4 LPS. After 4 or 18 hr of incubation, the culture medium was harvested and analyzed for cytokine content with specific immunoassays. The experiment was performed in triplicate and the results are presented as the mean and SEM. For IL-1α and IL-1β, the activity presented is the sum of that present in the culture medium and in the cell lysate.