Fig. 2
From: Distinct Roles of Synapsin I and Synapsin II during Neuronal Development
Immunocytochemical detection of synapses in hippocampal neurons from wild-type mice and from synapsin II and synapsin I/II double knockout mice. Hippocampal neurons obtained from wild-type (A–D), synapsin II (E–H), and synapsin I/II double knockout (I–L) mice were fixed after 4 (A, B, E, F, I, J) or 7 (C, D, G, H, K, L) days in culture. The cells were double stained with antibodies against tubulin (A, C, E, G, I, K) and synaptophysin (B, D, F, H, J, L). Note the absence of synaptophysin immunoreactive spots at 4 days in neurons from synapsin II and synapsin I/II double knockout mice. Scale bar: 20 µm.