Fig. 3
From: Receptors for Advanced Glycosylation Endproducts in Human Brain: Role in Brain Homeostasis
Cell surface AGE-specific binding proteins on human astrocytic U87MG cells. (A) Western blot of U87 MG cell membrane fractions using anti-AGE-R1, -R2, and -R3 antibodies (lanes 1–3, respectively). Lane 4 is a 125I-AGE-ligand blot on an identical cell membrane fraction as in lanes 1–3. Two main AGE-binding species of ~50 kD and ~ 30 kD, respectively, correspond to the size of AGE-R1 and AGE-R3. (B) 125I-AGE-BSA binding to the ~50 kD-species (lane 1) is inhibitable by anti-AGE-R1 (lane 2, arrow). (C) 125I-AGE-BSA binding to the ~30 kD (lane 1) is attenuated by anti-AGE-R3 antibody (lane 2, arrow).